5-second rule science fair project
Objective
In this 5-second rule experiment, we will learn about the 5-second rule science fair project. Find out if cleaning up dropped food in less than five seconds prevents germs from being transferred from the soil.
Material required
● Foods that will be evaluated (e.g., wet items like lunch meat and dry items like a jelly bean). Each item tested will require six copies.
● Swabs that are sterile
● Gloves that are sterile
● Timer
● Prepared nutrient agar in sterile plates
● Keep a notebook to keep track of your progress.
5-second rule Procedure
1. Using nourishing agar, make 12 sterile plates.
2. Wear a pair of sterile gloves (do not touch any non-living surface while wearing gloves).
3. Now, need to select two types of ground surfaces.
4. Discard the first test object (a piece of lunch meat, for example) onto the first sort of ground.
5. Kindly Set the timer
6. Remove the object from the ground after five seconds.
7. Using a sterile swab, clean the object (do not touch anything else with the swab).
8. The top of the petri dish should be removed. Keep your hand away from any non-living surfaces when holding the top of the bowl.
9. Slowly move the swab back and forth on the agar plate’s surface in a zigzag manner. No area of the agar should be touched twice.
10. Return to the Petri dish’s top and label it.
11. Remove your gloves and replace them.
12. Replace the first item in the test (for example, a new piece of lunch meat).
13. Repeat the test twice more with a new object each time (for example, two different jelly beans).
14. Change the soil types and test each thing twice.
15. Items that haven’t been tossed on the ground should be swabbed. For each item tested, make two “control” agar plates.
16. Maintain a temperature of 37°C for Petri dishes. At room temperature, bacteria take longer to proliferate. Ensure that all of the Petri dishes are stored together.
17. Time intervals of 24 hours, 36 hours, 48 hours, 60 hours, and 72 hours are used to photograph plates.
18. On each plate, count the number of bacterial colonies at each location.
Conclusion
The evidence in front of me demonstrated that the results of dry food are not equivalent to those of wet food. This knowledge can assist everyone in gaining a better understanding of germs and how they might harm us.
It may even assist us in becoming ill. We have a higher chance of avoiding becoming sick if we don’t pick up food off the ground.
The goal of this study is to see if the 5-second rule holds true for dry food on the floor.